JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972737.1
Database:	nt	Go to original record
Description:	Dryobalanops rappa isolate KASdr1 tRNA-Thr (trnT) gene, partial sequence; and trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; plastid.
Taxon:	Dryobalanops rappa
Original size:	1881 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1009	1591	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaagg
101   gcaatcctga gccaaatcct gtttaaaaaa gcgttcaaaa agaaatagga
151   taggtgcaga gactcaatgg aagttgttcg aacaaatggg gttgaccgct
201   tttgcgttgg taaaagaata cttctatcga aacttcagca aggataaaca
251   tatagaatac gtaatgaaaa attatctcaa aaaaaaggac tgggacacct
301   attcttttga tttctatttt tttataggtt atagcaaaaa gaattatatg
351   aaaaatcaaa agaatcgttg tgaatcgatt tcaaattgaa gaaagaatcg
401   aatagaatat tcattaataa aaccattcac tccatagtct gataaatcaa
451   tgattaattg gacgagaata aagatagagt cccattctac atgtcaatat
501   caataccgac aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details