JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972720.1
Database:	nt	Go to original record
Description:	Dipterocarpus cf. verrucosus JH-2017 isolate 05-1711 trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; plastid.
Taxon:	Dipterocarpus cf. verrucosus JH-2017
Original size:	1952 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	976	1582	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactgag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaaaaa agcgttcaaa aagaaatagg
151   ataggtgcag agactcaatg gaagttgttc gaccaaatgg ggttgactgc
201   ttttgcgttg gtaaaagaat acttctatcg aaatttcagc aaggakaaac
251   atataaacat atagaatacg taatgaaaaa cgatctcaca aaagacgact
301   gggacaccta ttcttttgat ttctattttt ttataggtta tagcaaatag
351   gttatagcaa aaagaattat aggaaaaatc aaaagaatcc ttgtgaatcg
401   atttcaaatt gaagaaagaa tcgaatagaa tattcattaa tcaaaccatt
451   caccccatag tctgataaat caatggatta attggacgag aataaagata
501   gagtcccatt ctacatgtca atatcaatac cgacaacaat gaaatttata
551   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details