JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972713.1
Database:	nt	Go to original record
Description:	Cotylelobium burckii isolate KAScb1 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Cotylelobium burckii
Original size:	2058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1038	1616	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   agggcaatcc tgagccaaat cctgtttaaa aaagcgttca aaaagaaata
151   ggataggtgc agagactcaa tggaagttgt tcgaacaaat ggggttgact
201   gcttttgcgt tggtaaaaga atacttctat cgaaacttca gccaggataa
251   acatatagaa tacataatga aaaaactatt tcaaaaaaga cgactgggac
301   acctattctt ttgatttttc atataattct ttttgctata acctataaaa
351   aaatagaaat caaaagaata ggtgtgaagc gattccaaat tgaagaaact
401   agtattcact aatcaaacaa ttcactccat agtctgataa atcaatggat
451   taattggacg agaataaaga tagagtccca ttctacatgt caatatcaat
501   accgacaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details