JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972712.1
Database:	nt	Go to original record
Description:	Cistus laurifolius isolate JH273 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Cistus laurifolius
Original size:	2454 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1410	2001	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agcaggtcaa aaagcaataa
151   aaaataggat aggtgcagag actcaatgga agttattcaa acaaatggga
201   ttgaatggct ttgcgttggt aaaagaatac ttctatactt ctatcaaaac
251   tcaagcaagg ataaacatat aaaataaact atttcaagaa aaaaaaatgg
301   ggtacctatt cttttgattt actttataag ttgtagcaaa aataatttta
351   tacaaaataa aaagaatcat tctgaatcca ttgcaagttg aagaatagaa
401   tatttattaa tcaaaaagaa aaccattcac tccataatct gataaatctt
451   ttgaaaaaca gattaatcgg acgagaataa agatagagtc ccgttctaca
501   tgtcaatatc aataccgaca acaatgaaag ttatagtaag aggaaaatcc
551   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details