JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972704.1
Database:	nt	Go to original record
Description:	Anisoptera laevis isolate 16-2455 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Anisoptera laevis
Original size:	2084 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1048	1633	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaaaaa agggttcaaa aagaaatagg
151   ataggtgcag agactcaatg gaagttgttc gaacaaatgg ggttgactgc
201   ttttgcgttg gtaaaagaat acttctatcg aaacttcagc aaggataaac
251   atatagaata cataatgaaa aaccgatttc aaaagagacg actgggacac
301   ctattctttt gatttctatt tttttatagg ttatagcaaa aagaattata
351   tgaaaaatca aaagaatcgt tgtgaatcga tttcaaattg aagaaagaat
401   caaatagaat attcactaat caaacaattc actccatagt ctgataaatc
451   aatggattaa ttggacgaga ataaagatag agtcccattc tacatgtcaa
501   tatcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details