JRC GMO-Amplicons | EURL GMFF

Record ID:	KY931664.1
Database:	nt	Go to original record
Description:	Parasponia rigida isolate PriWU20x00 chloroplast, partial genome.
Taxon:	Parasponia rigida
Original size:	132286 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49600	50189	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   atggggcaat cctgagccaa atccggtttt ctgaaaacaa acaaggattc
151   agaaaacgat aataaaaaag aataggatag gtgcagagac tcaatggaag
201   ctgttctaac aaacggagtt ggctgcgttg cgttaataaa ggaatccctt
251   tcatcgaaac tccagaaagg atgaagaata aacgcatata cgtacaaaaa
301   tcctatctcc aaataattaa tgaaaacccg aatccgtatt ttttttttat
351   tttcatgaaa aaggaaagaa ttgttgtgaa tcaattttaa gttgaaaaac
401   gaatcgaata ttcattgatc aaatcattta ctccatcaaa atctgataga
451   tcttttgaag acttgattaa tcggatgaga ataaagatag agtcccattc
501   tacatgtcaa tattgataag aatgaaattt atagtaagag gaaaatccgt
551   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details