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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KX388151.1
Database: nt Go to original record
Description: Cloning vector pCAMBIA1300DM_OsU6_AarI_Cas9, complete sequence.
Taxon: Cloning vector pCAMBIA1300DM_OsU6_AarI_Cas9
Original size: 14640 bp

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Method: QL-ELE-00-023 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of T35S pCAMBIA sequences (Rischitor et al., 2016).
Target:T35S pCAMBIA

View other 1686 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 9821 9957 - 137 bp
Amplicon: 137 bp 
1     cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
51    ttattgatag aagtatttta caaatacaaa tacatactaa aggtttctta
101   tatgctcaac acatgagcga aaccctatag gaaccct
Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.06e-09
primerB 100.0 23 bp 0 0 137 115 1.68e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.