Match details
| Record ID: |
KY000046.1 |
| Database: |
nt |
Go to original record |
| Description: |
Agrobacterium genomosp. 1 strain CFBP2177 plasmid pTi_CFBP2177, complete sequence. |
| Taxon: |
Agrobacterium tumefaciens |
| Original size: |
178205 bp |
View other 6 matches for this record
View other 3921 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 2 |
0 |
20305 |
20398 |
- |
94 bp |
|
1 aagcacatat gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51 cactatcagc tagcaaatat ttcttgtcaa aaatgcacca ctga
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
96.2 |
26 bp |
1 |
0 |
1 |
26 |
1.41e-10 |
| primerB |
95.8 |
24 bp |
1 |
0 |
94 |
71 |
1.65e-09 |
| probe |
100.0 |
26 bp |
0 |
0 |
28 |
53 |
3.03e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
