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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KX510274.1
Database: nt Go to original record
Description: Expression vector hbt::LIC-HA, complete sequence.
Taxon: Expression vector hbt::LIC-HA
Original size: 3592 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 2820 3014 + 195 bp
Amplicon: 195 bp 
1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca caaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 21 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.44e-06
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.