JRC GMO-Amplicons | EURL GMFF

Record ID:	MF035991.1
Database:	nt	Go to original record
Description:	Monachather paradoxus isolate Lazarides149 chloroplast, complete genome.
Taxon:	Monachather paradoxus
Original size:	137652 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48978	49592	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaacccggg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgttgg tagtgaaacc cctctaaatt
251   ggagaaagaa gggctttata catctaatac acacgtatag atactgacat
301   agcaaacgat taatcacaga acccatatca taatataggt tctttattta
351   tttatttttt agaatgaaat taggaatgat tatgaaatag aaaattcata
401   atttttttga attattgtga atccatttca atcgaatatt gagtaatcaa
451   atccttcaat tcattgtttt tgagatcttt taaaaagtag attaatcgga
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttataagttg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details