JRC GMO-Amplicons | EURL GMFF

Record ID:	MF035990.1
Database:	nt	Go to original record
Description:	Monachather paradoxus isolate Columbus5120 chloroplast, complete genome.
Taxon:	Monachather paradoxus
Original size:	137521 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48862	49477	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaacccggg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgttgg tagtgaaacc ccctctaaat
251   tggagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccatatc ataatatagg ttctttattt
351   atttattttt tagaatgaaa ttaggaatga ttatgaaata gaaaattcat
401   aatttttttg aattattgtg aatccatttc aatcgaatat tgagtaatca
451   aatccttcaa ttcattgttt ttgagatctt ttaaaaagta gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtt gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details