JRC GMO-Amplicons | EURL GMFF

Record ID:	MF101229.1
Database:	nt	Go to original record
Description:	Afgekia mahidolae tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Afgekia mahidolae
Original size:	1059 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaaaaaaaa aaaagaaaag
151   ttcagaaagc gagaatcaaa aaaggatagg tgcagagact caatggaagc
201   tgttctaaaa aatggagttg acgacatttc cttacgtagt aggaaagaat
251   caaggataaa tatttatata tatatatata taaatatata tatgtactga
301   aatactattt caattgatta atgaagactc caaacttcta tttttaaata
351   tttctatcat aaaagaaaga tttgaatcaa atcaattaca agttgtaaaa
401   aatattggaa tattcattga ttaaatcaat aactccatca tagtctgatg
451   gatcttttga agaactgatt aatcagacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca ccaatgaaat ttttagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details