JRC GMO-Amplicons | EURL GMFF

Record ID:	MF101218.1
Database:	nt	Go to original record
Description:	Pyrgophyllum yunnanense isolate BMPK47 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pyrgophyllum yunnanense
Original size:	978 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtaaggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aatttaaaat
101   gggcaatcct gagccaaatc cttagtttga taaaccttag ttttatcaaa
151   ctagaataaa aaaaggatag gtgcagagac tcgatggaag ctgttctaac
201   gaatgaagtt gactacgttt cgttggtagt tggaatccat ctatcaaaat
251   tacagaaaag atgttcctat atacctaata catacgtata catactgaca
301   tatcaaatca aacgattaat catgactcga atccattata ttatatggat
351   aattataata ttaaaaattc agaattagag ttattgtgaa tccagtctcc
401   aatggaagtt gaaagaagaa ttgaatattc aattcaatta ttaaatcatt
451   cattccagag tttgatagat cttttgaaaa actgattaat cggacgagaa
501   taaagagaga gtcccattct acatgtcaat gccgacaaca atgaaatttt
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details