JRC GMO-Amplicons | EURL GMFF

Record ID:	MF101217.1
Database:	nt	Go to original record
Description:	Caulokaempferia saxicola isolate BMPK30 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Monolophus saxicola
Original size:	1000 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	608	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtaaggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattttaaat
101   gggcaatcct gagccaaatc cttagtttga taaaactaag gtttatcaaa
151   ctagaataaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatgaagt tgactacgtt tcgttggtag ttggaatccg tctatcaaaa
251   ttacagaaaa gatgttccta tatacctaat acatacgtat acatactgac
301   atatcaaatc aaacgattaa tcatgactcg aatccattat attatatgga
351   taattataat atgaaaaatt cagaattaga gttattgtga atccagtcca
401   atggaagttg aaagaagaat ttaatattca attcaattat taaatcattc
451   attccagagt ttgatagatc tttttaaaaa ctgattaatc ggacgagaat
501   aaagagagag tcccattcta catgtcaata ccgacaacaa tgaaatttat
551   agtaagagga aaatccgtcg actttagaaa tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details