JRC GMO-Amplicons | EURL GMFF

Record ID:	MF101216.1
Database:	nt	Go to original record
Description:	Caulokaempferia limiana isolate BMPK32 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Monolophus limianus
Original size:	993 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtaaggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatttaaaat
101   gggcaatcct gagccaaatc cttagtttga taaacctttt ttttatcaaa
151   ctagaataaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatgaag ttgactacgt ttcgttggta gttggaatcc gtctatcaaa
251   attacagaaa agatgttcct atatacctaa tacatacgta tacatactga
301   catatcaaat caaacgatta atcatgactc gaatccatta tattatatgg
351   ataattataa tatgaaaaat taagaattag agttattgtg aatccagtcc
401   aatggaagtt gaaagaagaa ttgaatattc aattcaatta ttaaatcatt
451   cattccagag ttagatagat cttttgaaaa actgattaat cggacgagaa
501   taaagagaga gtcccattct acatgtcaat accgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details