JRC GMO-Amplicons | EURL GMFF

Record ID:	MF061222.1
Database:	nt	Go to original record
Description:	Palmerella debilis subsp. serrata plastid, complete genome.
Taxon:	Palmerella debilis subsp. serrata
Original size:	164787 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	52730	53312	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtagggaa
51    acttactaag tgagaacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcccgtttt ctgaaaacaa ggttcagaaa
151   gcgaaaatca aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gattgtctta cgttggtaaa ggaatctttc tcttgaaact
251   tccgaaagag tgaaagataa ccttgtataa tatacatagg taaagtatgc
301   gtactgaaat actataaaat atcaaatgat taatgacgac tcgaacctgt
351   atttgttata tgaaaaatgg aagaattctt gtgaatcgat tcagattgaa
401   gaatgaagag acaaacaaat cgttcactcc agagtctgat agatcttttt
451   aagaattgag tcattggacg agaataaaga tagagtccca ttctacatgt
501   caatattggc aacaatgcaa tttatagtaa gaggaaaatc cgtcgatttt
551   agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details