JRC GMO-Amplicons | EURL GMFF

Record ID:	KY596157.1
Database:	nt	Go to original record
Description:	Pseudosorghum fasciculare chloroplast, complete genome.
Taxon:	Pseudosorghum fasciculare
Original size:	140538 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48951	49551	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc caaatcccct tttttgaaaa aacaagtggt
151   tctcaaacta gaacccaaag gaaaaggata ggtgcagaga ctcaatggaa
201   gctgttctaa cgaatcgaag taataacgat taatcacaga acccatatta
251   taatatatgg ttctttattt tatttttaga atgaaattag gaatgattat
301   gaaatagaaa attcataatt tttttagaat tattgtgaat ctattccaat
351   cgaattagga atgattatga aatagaaaat tcataatttt tttagaatta
401   ttgtgaatct attccaatcg aatattgagt aatcaaatcc ttcaattcat
451   tgttttcgag atcttttaaa aagtggatta atcggacgag gataaagaga
501   gagtcccatt ctacatgtca atactgacaa caatgaaatt tctagtaaaa
551   ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details