JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815607.1
Database:	nt	Go to original record
Description:	Coptosperma supra-axillare voucher Mouly 621 (P) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Coptosperma supra-axillare
Original size:	1622 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	712	1277	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaggg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cattagtaga gaaatctttc catctaaaat tccgaaaggg
251   taaagtgaag gataaacgta tatatacgta ttgaatacta tgtcaaatga
301   ttaatgacga ctcgactgaa tctgtatttt ttatatgaaa acggaagaat
351   tggtgtgaat agattccaca ttgaagaaag aatcgaatat tcattgatca
401   aatgattcac tccatagtct gatagatctt ttcaagaatt tattaatcgg
451   acgagaataa agatagagtc ctgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details