JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815606.1
Database:	nt	Go to original record
Description:	Coptosperma peteri voucher Lovett & Congdon 2991 (MO) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coptosperma peteri
Original size:	1676 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	743	1305	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcattagtag agaaatcttt ccatctaaaa ttccgaaagg
251   gtaaagtgaa ggataaacgt atatatacgt attgaatact atgtcaaatg
301   attaatgacg actcgactga atctgtttta tatgaaaacg gaagaattgg
351   tgtgaataga ttccacattg acgaaagaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgat agatcttttc aagaattgat taatcggacg
451   agaataaaga tagagtcctg ttctacatgt caatgtcggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details