JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815593.1
Database:	nt	Go to original record
Description:	Coptosperma graveolens voucher Luke 9013 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coptosperma graveolens
Original size:	1676 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	740	1305	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    actcactaag tgataacttt caaattcaga gaaacctgga attaataaaa
101   aggggcaatc ctgagccaaa tcccgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaggg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cattagtaga gaaatctttc catctaaaat tccgaaaggg
251   taaagtgaag gataaacgta tatatacgta ttgaatacta tgtcaaatga
301   ttaatgacga ctcgactgaa tctgtatttt ttatatgaaa acggaagaat
351   tggtgtgaat agattccaca ttgaagaaag aatcgaatat tcattgatca
401   aacgattcac tccatagtcc gatagatctc ttcaagaatt gattaatcgg
451   acgagaataa agatagagtc ctgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details