JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815545.1
Database:	nt	Go to original record
Description:	Cremaspora triflora voucher Merello 1682 (MO) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cremaspora triflora
Original size:	1647 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	756	1320	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaacaaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatgtat tgaatactat atcaaatgat
301   taatgaggac tcgactgaat ctgtattttt tatatgaaaa cggaagaatt
351   ggtgtgaata gattccacat tgaagaaaga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details