JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815514.1
Database:	nt	Go to original record
Description:	Gardenia fiorii voucher SMP 209 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Gardenia fiorii
Original size:	1512 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	603	1177	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat tctgagccaa atcccatttt ccgaaaccaa aggtgaaaaa
151   gggataggtg cagagactca acggaagctg ttctaacaaa tggagttggc
201   tgcattagta gagaaatctt tccatcgaaa attccgaaag tataaagtga
251   aggataaacg taaagtataa agtgaaggat aaacgtatat atacgtattg
301   aatactatat caaatgatta atgacgactc gactgaatct gtatttttta
351   tatgaaaacg gaagagaata gattccacat tgaagaaaga atcgaatatt
401   cattgatcaa atgattcact ccatagtctg atagatcttt tcaagaattg
451   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaatgtcg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details