JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815493.1
Database:	nt	Go to original record
Description:	Rothmannia urcelliformis voucher Luke 9052 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Rothmannia urcelliformis
Original size:	1742 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	806	1374	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccacacta agtgataact ttcaaattca gagaaaccct ggaattaata
101   aaaaggggca atcctgagcc aaatcccgtt ttccgaaacc aaaggttcag
151   aaagtgaaaa aaggataggt gcagagactc aacggaagct gttctaacaa
201   atggagttgg ctgcgttagt agagaaatct ttccatctaa aattccgaaa
251   ggataaagtg aaggataaac gtatatatac gtattgaata ctatatcaaa
301   tgattaatga cgactcgact gaatctgtat tttttatgtg aaagcggaag
351   aattggtgtg aatagattcc acattgaaga aagaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgatagat cttttcaaga attgattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gtcggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details