JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815482.1
Database:	nt	Go to original record
Description:	Rothmannia engleriana voucher Luke 9988 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Rothmannia engleriana
Original size:	1629 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	725	1286	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcctt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataacgta tatatacgta ttgaatacta tatcaaatga
301   ttaatctgaa tctgtatttt ttttttttat gtgaaaacag aagaattggt
351   gtgaatagat tccacattga agaaagaatc gaatattcat tgatcaaacg
401   attcactcca tagtctgata gatcttttca agaattgatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aatgtcggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details