JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815460.1
Database:	nt	Go to original record
Description:	Tricalysia acocantheroides voucher Lantz 121 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tricalysia acocantheroides
Original size:	1709 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	775	1339	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaacctgga attaataaaa
101   aggggcaatc ctgagccaaa tcccgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat tccgaaagga
251   taaagtgaag gataaacgta tatacgtact gaatactata tcaaatgatt
301   aatgacgact cgactgaatc tgtatttttt atataaaaac ggaagaattg
351   gtgtgaatag attccacatt gaagaaagaa tcgaatattc attgatcaaa
401   tgattcactc catagtcctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details