JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815452.1
Database:	nt	Go to original record
Description:	Empogona bequaertii voucher Walters et al. 942 (MO) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Empogona bequaertii
Original size:	1696 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	763	1325	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat tccgaaagga
251   taaagtgaag gataaacgta tatacgtatt gaatactata tcaaatgatt
301   aatgacgact cgactgaatc tgtattttta tataaaaacg gaagaattgg
351   tgtgaataga ttccacattg aagaaagaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgat agatcttttc aagaattgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatgtcggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details