JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815446.1
Database:	nt	Go to original record
Description:	Coffea semsei voucher Luke 9260 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coffea semsei
Original size:	1700 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	766	1340	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggaaaggtt
151   cagaaagtga aaaaaaaaaa ggataggtgc agagactcaa cggaagctgt
201   tctaacaaat ggagttggct gcgttagtag agaaatcttt ccatctaaaa
251   ttccgaaagg ataaagtgaa ggataaacgt atatacgtat tgaatactat
301   atcaaatgat taatgacgac tcaactgaat ctggattttt tatataaaaa
351   tggaagaatt ggtgtgaata gattccacat tgaagaaaga atcgaatatt
401   cattgatcaa atgattcact ccatagtctg atagatcttt tcaagaattg
451   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaatgtcg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details