JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815428.1
Database:	nt	Go to original record
Description:	Coffea mangoroensis voucher Razakamalala RZK 6506 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coffea mangoroensis
Original size:	1707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	769	1337	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggaaaggtt
151   cagaaagtga aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tggctgcgtt agtagagaaa tctttccatc taaaattccg
251   aaaggtaaag tgaaggataa acgtatatac gtattgaata ctatattaaa
301   tgattaatga cgactcaact gaatctgtat tttttatata aaaatggaag
351   aattggtgtg aatagattcc acattgaaga aagaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgatagat cttttcaaga attgattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gtcggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details