JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815416.1
Database:	nt	Go to original record
Description:	Diplospora dubia voucher Kainulainen et al. 23 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Diplospora dubia
Original size:	1680 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	749	1312	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cgggagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt atataaaaac ggaagaattg
351   gtgtgaatag attccacatt gaagaaagaa tcgaatattc attgatcaaa
401   tgattcactc catagtctga tagatctttt caagaattga ttaatcggac
451   gagaataaag atagagtccc gttctacatg tcaatgtcgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details