JRC GMO-Amplicons | EURL GMFF

Record ID:	KY040019.1
Database:	nt	Go to original record
Description:	Delonix decaryi voucher Capuron 28520-SF (K) trnL gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence.
Taxon:	Delonix decaryi
Original size:	1014 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaacccc agaaaagaaa ggatcaagga tgaacatacg tatagtactg
301   aaatactatt tcaattgatt agaccagaca gaccccaaat ctctattttt
351   tcatatttat atgagaaatg aaagatgtga atcgattcca agttgaagaa
401   agaatcgaat attcattgat caaatcattc actccatcat agtctgatag
451   atcttttcaa gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtta ataccggcaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

View other 9 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details