JRC GMO-Amplicons | EURL GMFF

Record ID:	KX510115.1
Database:	nt	Go to original record
Description:	Lampranthus spectabilis tRNA-Thr (trnT-UGU) gene, partial sequence; tRNA-Leu (trnL-UAA) gene, complete sequence; and tRNA-Phe (trnF-GAA) gene, partial sequence; chloroplast.
Taxon:	Lampranthus spectabilis
Original size:	1791 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	779	1388	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaatag gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaactcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaac tccctttttc aaaagaaaaa aaagaagaaa
151   gcaagaaaaa aggataggtg cagagactca aaggaagcta ttctaacaaa
201   tgggattgac tgcgttgtta taagacttat tccatcaaaa ctccaataaa
251   aagggggtga aacgtatacg tacttaacta tgttaaatga ttaatgacaa
301   cccgaatctt tatctttctt tttttttttc taatttttct tttttttttt
351   tacgaatatg aaatcaaaat ttttatatga gaaaagggaa gaattattgt
401   gaatcgattc gaagttgaaa aaagaatcaa atatgcattc atcaaagcat
451   tcactccata gtctgatagt tcttttgaag aacttattaa tcggacgaga
501   atgaagatag agtcccgttc tacatgtcaa tactgacaac aatgaaattt
551   atagtaagag gaaaatccgt cgactttcaa aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details