JRC GMO-Amplicons | EURL GMFF

Record ID:	KY174338.1
Database:	nt	Go to original record
Description:	Carpinus tientaiensis chloroplast, complete genome.
Taxon:	Carpinus tientaiensis
Original size:	160104 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	51591	52196	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccc aaaacaaata aaacaaattt
151   aagggttcat aaagcgagaa taaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaattgga gttgacttcg ttccgttagt aaagtaatcc
251   ttctatcaaa actacagaaa ggatgaagga taaccgtata tacatacgta
301   tacgtcctga aatactatct caaaaaatga atgacgaccc gaatctttat
351   ttcttttttt atatttatat aaaaactgaa tgaaagagtt ggtgtgaatc
401   gatccaaatt gaagaaagaa tcgaatattt attaatcaaa ttagttactc
451   cgcatagtct gatagatctt ttgaagaact aattaatcgg acgagaataa
501   agatagagtc ccattctaca tgtcaatacc gacaacaatg aaatttatag
551   taagaggaaa atccgtcgac tttataaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details