JRC GMO-Amplicons | EURL GMFF

Record ID:	KF359917.1
Database:	nt	Go to original record
Description:	Oryza brachyantha chloroplast, complete genome.
Taxon:	Oryza brachyantha
Original size:	134644 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46881	47460	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccgtgttttg agaaaaaagg ataggtgcag
151   agactcaatg gaagctgttc taacgaatcg agttaattac gttgtgttgg
201   tagcggaact cccttctaaa ttagagaaag aagggctttc gaaatctaat
251   acacacgtat agatactggc atagcaaacg attaatcaca gaactcatat
301   cataatatag gttctttaat tctattttag aatgaaaata ggaatgatta
351   tgaaatagaa aattcataat ttttttagaa ttgttgtaaa tccattccaa
401   tcgaatattg agtaatcact tcaattcata gttttcgaaa tcgattaaaa
451   agtggattaa tcggacgagg ataaagagag agtcccattc tacatgtcaa
501   tactgacaac aatgaaattt ctagtaaaag gaaaatccgt cgactttata
551   agtcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details