JRC GMO-Amplicons | EURL GMFF

Record ID:	KF359908.1
Database:	nt	Go to original record
Description:	Oryza punctata chloroplast, complete genome.
Taxon:	Oryza punctata
Original size:	134582 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46397	47012	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccgtgttttg agaaaacaag cggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagggaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta aaatgaaaat aggaatgatt atgaaatttt ctatttcata
401   atttttttag aattgttgtg aatccattcc aatcgaatat tgagtaatca
451   aatccttcga ttcatagttt tcgaaatctt tttaaaactg gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttctaagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details