JRC GMO-Amplicons | EURL GMFF

Record ID:	KY656311.1
Database:	nt	Go to original record
Description:	Agrimonia repens isolate Chung 090708(OKL) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Agrimonia repens
Original size:	943 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	545	+	545 bp

Amplicon:

545 bp

1     cgaaatcggt agacgctacg gacttaaatt gaattgagcc ttggtatgga
51    aacctaccaa gtgataactt tcaaattcag agaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cccgttttat gaaaacaaac aagggtttca
151   caaagcgaga aaaaataaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaggtg gagttggctg cagtgtgttc gtaaaggaat ccttccatcg
251   aaacttccga aaggatgaaa gataaacata ttaagtatat gtatatttac
301   tgaaatacta tagccaaatg attcaaaatt caaaatttaa aaataaaaag
351   aattaattat tcattgatca aataattcac tccattatag tctgataaat
401   ctttttattt ttaataatta ttaatcggat taagaataaa gatagagtcc
451   cattctacat gtcaatatcg acaacaatga aatttatagt aataggaaaa
501   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	545	526	2.45e-08

Match details