JRC GMO-Amplicons | EURL GMFF

Record ID:	KY697465.1
Database:	nt	Go to original record
Description:	Viola reichenbachiana trnL gene, intron; chloroplast.
Taxon:	Viola reichenbachiana
Original size:	577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	576	-	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggtaatcc tgagccaaat ccggtttttt gaaatgaaaa aaagtttata
151   tagacagaat aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   ataaatggag ttgactgtct tgcattagta aagtaaggga acccttcgtt
251   aaaattccgg attcaaagta aaggataacc ctataaaata catatacgta
301   ctgaaagact atctcaaata attaatgtaa tgatgacccg aactcagtat
351   ttatatatat ttatatgaaa aataaaaata ttgaatcgat ttcaagttga
401   agaaaaaatc gattgatcaa atcattcact ccacagtctg atagataact
451   aattaatcgg acaagaataa agatagagtc ccattctaca tgttaatatc
501   gacaacaagg aaatttatag taagaggaaa atccgtcgac tttttaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details