Match details
Record ID: |
LC221337.1 |
Database: |
nt |
Go to original record |
Description: |
Dual site Gateway binary vector pGWB6400-MD8-Pnos-NY2 DNA, complete sequence. |
Taxon: |
Dual site Gateway binary vector pGWB6400-MD8-Pnos-NY2 |
Original size: |
13199 bp |
View other 16 matches for this record
View other 1572 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
6225 |
6387 |
- |
163 bp |
|
1 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
51 tctgcaccgg atctggatcg tttcgcatga ttgaacaaga tggattgcac
101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
151 acaacagaca atc
View other 978 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
23 bp |
0 |
0 |
1 |
23 |
2.01e-10 |
primerB |
100.0 |
22 bp |
0 |
0 |
163 |
142 |
6.81e-10 |
probe |
100.0 |
25 bp |
0 |
0 |
140 |
116 |
1.74e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.