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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HE582394.1
Database: nt Go to original record
Description: Cloning vector pAJM-EPRV
Taxon: Cloning vector pAJM-EPRV
Original size: 6299 bp

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 311 476 + 166 bp
Amplicon: 166 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
51    tctgcccgga tccgggggga tcgtttcgca tgattgaaca agatggattg
101   cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
151   ggcacaacag acaatc
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.05e-10
primerB 100.0 22 bp 0 0 166 145 6.94e-10
probe 100.0 25 bp 0 0 143 119 1.77e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.