JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ662221.1
Database:	nt	Go to original record
Description:	Gaillonia yemenensis trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Plocama yemenensis
Original size:	1809 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	777	1385	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaacaa aggtttagaa
151   agtgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcgttagtc gagaagtctt tccatcaaaa attcagaaag
251   tataaggatt aaggatgaag tgaaggataa acggatatac tatccatacg
301   tagtgaatag tatattaaat gagtaatgac aaaatgagta atgacagcca
351   aaatgaatct atattctatt ttttctataa aaaataaaga attgttgtta
401   atcgattcca cgtttaagaa agaagcgaat attcatttat caaatgattc
451   actccatagt ctgctagatc ttttcacgaa ctgattaatc ggacgagaat
501   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
551   tgttaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details