Match details
Record ID: |
KY464890.1 |
Database: |
nt |
Go to original record |
Description: |
Nicotiana benthamiana transgenic NptII (aph(3')-II (or nptII)) and modified green fluorescent protein mGFP5-ER genes, complete cds; and transposon Tn5393 TnpA (tnpA) and TnpR (tnpR) genes, complete cds. |
Taxon: |
Nicotiana benthamiana |
Original size: |
9484 bp |
View other 45 matches for this record
View other 1572 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
1781 |
1943 |
+ |
163 bp |
|
1 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
51 tctgcaccgg atctggatcg tttcgcatga ttgaacaaga tggattgcac
101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
151 acaacagaca atc
View other 978 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
23 bp |
0 |
0 |
1 |
23 |
2.01e-10 |
primerB |
100.0 |
22 bp |
0 |
0 |
163 |
142 |
6.81e-10 |
probe |
100.0 |
25 bp |
0 |
0 |
140 |
116 |
1.74e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.