JRC GMO-Amplicons | EURL GMFF

Record ID:	KX094399.1
Database:	nt	Go to original record
Description:	Amaranthus tricolor chloroplast, complete genome.
Taxon:	Amaranthus tricolor
Original size:	150027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47532	48135	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaaaaaaa
151   aataaggatt cagaaagcaa gaataaaaaa aaggataggt gcagagactc
201   aaaggaagct gttctaacaa atggagttga ttgtgctggg ttgttcgaag
251   aattcttctg tcgaaattcc tgattaatgg cacttcgaat ctattctgta
301   tttctatttt tagaattttt agatatttct gatttcgaga taatatagaa
351   tatgaaattt tctagaaata aaaatgaaaa aatggaagaa tttttttgac
401   tctatttcaa gttgaaaaaa gaatcaaatc attcactcca tagtctgata
451   gatctttctt tgaaagaacc ggttaatcgg ttactcggac gagaataaag
501   atagagtccc attatacatg tcaatactga caacaatgaa atttatagta
551   cgaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
601   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details