JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ662170.1
Database:	nt	Go to original record
Description:	Spermacoce filituba trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Spermacoce filituba
Original size:	1830 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	798	1385	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccactgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctatttt ccgaaaacaa aaaaggggtt
151   cataaagtga aaaaggggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttgggtgcgt tagtcgataa gtctttccag gaaaaattcc
251   ttaagtagtt aaggataaag cgaaggataa acgtatatac atacgtagtg
301   aatagcatat taaatgagta ctgacagctc aaactaatct ctattttttc
351   tatgaaaaaa gaaagaattg ttgttaatag attctatgtt taagaaagaa
401   tcgaatatgc attgatcaaa tgattcactc catagtctga tagatctttt
451   cacgaactga ttaatcagac gagaataaag atagagtccc atttttacat
501   gtcaattccg gcaacaatga aatttatagt taataggaaa atccgtcgac
551   tttttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details