JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ662163.1
Database:	nt	Go to original record
Description:	Pseudogaillonia hymenostephana trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Plocama hymenostephana
Original size:	1738 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	721	1315	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggtttagaa
151   agtgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgccttagtc gagaagtctt tccatcaaaa attcagaaag
251   tataaggatt aaggatgaag tgaaggataa acggatatac tatccatacg
301   tagtgaatag tatattaaat gagtaatgac agccaaaatg aatctatatt
351   ctattttttc tataaaaaaa taaggaattg ttgttaatcg attccacgtt
401   taagaaagaa tcgaatattc atttatcaaa tgattcactc catagtctgc
451   tagatctttt cacgaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaatgccgg caacaatgaa atttatagtt aagaggaaaa
551   tccgtcgact tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details