JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ662143.1
Database:	nt	Go to original record
Description:	Gaillonia tinctoria trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Plocama tinctoria
Original size:	1777 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	739	1337	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aataaaaagg ggcaatcctg agccaaatcc tattttccga aaacaaaggt
151   ttagaaagtg aaaaagggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg ttagtcgaga agtctttcca tcaaaaattc
251   agaaagtata aggattaagg atgaagtgaa ggataaacgg atatactatc
301   catacgtagt gaatagtata ttaaatgagt aatgacagcc aaaatgaatc
351   tatattctat tttttctata aaaaataaag aattgttgtt aatcgattcc
401   acgtttaaga aagaagcgaa tattcattta tcaaatgatt cactccatag
451   tctgctagat cttttcacga actgattaat cggacgagaa taaagataga
501   gtcccattct acatgcaata ccggcaacaa tgaaatttat tgttaagagg
551   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details