JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ662140.1
Database:	nt	Go to original record
Description:	Gaillonia crocyllis trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Plocama crocyllis
Original size:	1738 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	707	1300	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggtttagaa
151   agtgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcgttagtc gagaagtctt tccatcaaaa attcagaaag
251   tataaggatt aaggatgaag tgaaggataa acggatatac tatccatacg
301   tagtgaatag tatattaaat gagtaatgac agccaaaatg aatctatatt
351   ctattttttc tataaaaaat aaagaattgt tgttaatcga ttccacgttt
401   aagaaagaag cgaatattca tttatcaaat gattcactcc atagtctgct
451   agatcttttc acgaactgat taatcggacg agaataaaga tagagtccca
501   ttctacatgt caatgccggc aacaatgaaa tttattgtta agaggaaaat
551   ccgtcgactt tcaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details