JRC GMO-Amplicons | EURL GMFF

Record ID:	AM404253.1
Database:	nt	Go to original record
Description:	Calderonella sylvatica plastid tRNA-Leu gene (partial), trnL-trnF intergenic spacer and tRNA-Phe gene (partial), specimen voucher J. D. Dwyer 9383A
Taxon:	Zeugites sylvaticus
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	618	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggcatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctgttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgttgg gagtggaact ccctcgaaat
251   tagagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacgg aacccatatc ataatatagg ttctttattt
351   atttattttg tagaatgaaa ttaggaatga ttatgaaata gaaaattctg
401   cattttttta gaattattgt gaattcattc caatcgaata ttgagtaatc
451   aaatccttca attcattgtt ttcgagatct tttcaaaagt ggattaatca
501   gacgaggata aagagagagt cccattatac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttctaagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details