JRC GMO-Amplicons | EURL GMFF

Record ID:	AM404247.1
Database:	nt	Go to original record
Description:	Zeugites latifolia plastid tRNA-Leu gene (partial), trnL-trnF intergenic spacer and tRNA-Phe gene (partial), specimen voucher R. Torres 7374
Taxon:	Zeugites latifolia
Original size:	1017 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	618	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggcatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgttgg gagtggaact ccctcgaaat
251   tagagaaaga agggctttat acatctaata cacacgtata gatacttaca
301   tagcaaacga ttaatcacgg aacccatatc ataatatggg ttctttattt
351   atttattttg tagaatgaaa ttaggaatga ttatgaaata gaaaattctg
401   aattttttta gaattattgt gaatccattc caatcgaata ttgagtaatc
451   aaatccttca attcattgtt ttcgagatct tttcaaaagt ggattaatca
501   gacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttctaagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details