JRC GMO-Amplicons | EURL GMFF

Record ID:	AM404245.1
Database:	nt	Go to original record
Description:	Zeugites pittieri plastid tRNA-Leu gene (partial), trnL-trnF intergenic spacer and tRNA-Phe gene (partial), specimen voucher A. Soriano 518
Taxon:	Zeugites pittieri
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	619	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggcatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaaacaag tggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaggtaatta cgttgtgtcg ggagtggaac tccctcgaaa
251   ttagagaaag aagggcttta tacatctaat acacacgtat agatactgac
301   atagcaaacg attaatcacg gaacccatat cataatatag gttctttatt
351   tatttatttt gtagaatgaa attaggaatg attatgaaat agaaaattct
401   gaattttttt agaattattg tgaatccatt ccaatcgaat attcagtaat
451   caaatccttc aattcattgt tttcgagatc ttttcaaaag tggattaatc
501   agacgaggat aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttctaag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details