Match details
Record ID: |
EU124676.1 |
Database: |
nt |
Go to original record |
Description: |
Brassica napus transgenic cultivar Topas 19/2 3'-terminal transgene junction sequence. |
Taxon: |
Brassica napus |
Original size: |
823 bp |
View other 5 matches for this record
View other 1572 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
184 |
327 |
- |
144 bp |
|
1 ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
51 gatcatgtgg attgaacaag atggattgca cgcaggttct ccggccgctt
101 gggtggagag gctattcggc tatgactggg cacaacagac aatc
View other 3 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
23 bp |
0 |
0 |
1 |
23 |
1.77e-10 |
primerB |
100.0 |
22 bp |
0 |
0 |
144 |
123 |
5.98e-10 |
probe |
100.0 |
25 bp |
0 |
0 |
121 |
97 |
1.53e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.