JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ916886.1
Database:	nt	Go to original record
Description:	Zygostelma benthami voucher Middleton 849 (A) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Zygostelma benthamii
Original size:	1690 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	798	1362	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcgac ggaagatgtt ctaacaaatg
201   gagttggccg cgttggtaaa gaaatccttc catcgaaaat tcagaaagga
251   tgaaggataa acacatatac atacgtattg aatactatat caaatgatta
301   tgccgacccg aatgaatctg tattttttct ataaaaaacg gaagaattgg
351   tgtgagtcga ttctttcttc aatgtggaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgta gatccccttt tcaagaacgg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact taaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details